Paraphenylenediamine Hepatotoxic Effects in Adult Female Albino Rats

Paraphenylenediamine (PPD) is a chemical product used in hair dye preparations and temporary tattoos to fasten the dyeing process intensify its results. PPD can evoke local and/or systemic toxic effects either when utilized topically, inhaled or ingested. The aim of this study was evaluate on liver adult female albino rats. Thirty-two rats were divided into 3 groups. Group I (control group) 16 rats: which subdivided 2 equal subgroups: Subgroup IA (negative control) 8 received only regular diet water, IB (vehicle 1 ml distilled water once daily by oral gavage, 5 days/week for weeks. II (PPD treated Each rat 10 mg/kg B.W weeks gavage. III (Follow up followed two without treatment. After group & after follow III, subjected blood sample collection estimating AST, ALT ALP levels. Then livers dissected out three parts. first part oxidative stress markers MDA GSH. Second histopathological examination under light microscope. third examined comet assay determine DNA damage. results revealed that induced significant increase (AST, ALP), hepatic decrease It also several alterations damage cells, cessation treatment ameliorates damaging effects.